Hotspot

trnF-GAA-ndhJ

LSC · 47771-48331 · 561 bp

Back to Eriachne

Pi 0.0207

MarkerSeek score 74.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 68
pi: 0.0207
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	671-703 bp	1.0	88.1
trnF-GAA-ndhJ_p2	GATGCACAAGAGAGGGTCAG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	725-757 bp	1.0	88.0
trnF-GAA-ndhJ_p3	GAGGGTCAGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.9	57.9	714-746 bp	1.0	87.7
trnF-GAA-ndhJ_p4	GATGCACAAGAGAGGGTCAG	ATAGGCTGGCCCTTACGTAA	55.0	50.0	58.0	58.2	715-747 bp	1.0	87.5
trnF-GAA-ndhJ_p5	GGATGATGCACAAGAGAGGG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	729-761 bp	0.913043	83.1