Hotspot

trnL-UAA-trnF-GAA

LSC · 47352-47697 · 346 bp

Back to Eriachne

Pi 0.0187

MarkerSeek score 68.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 33
pi: 0.0187
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	58.4	58.1	369-455 bp	1.0	86.6
trnL-UAA-trnF-GAA_p2	GTTGTGAGGGTTCAAGTCCC	TTTGTTTGCCAGGAACCAGA	55.0	45.0	58.4	57.8	389-475 bp	1.0	85.5
trnL-UAA-trnF-GAA_p3	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	370-456 bp	1.0	84.6
trnL-UAA-trnF-GAA_p4	GTTGTGAGGGTTCAAGTCCC	TGTTTGCCAGGAACCAGATT	55.0	45.0	58.4	57.6	387-473 bp	1.0	83.7
trnL-UAA-trnF-GAA_p5	GTTGTGAGGGTTCAAGTCCC	TTGTTTGCCAGGAACCAGAT	55.0	45.0	58.4	57.6	388-474 bp	1.0	83.7