Hotspot

trnT-UGU-trnL-UAA

LSC · 46334-46733 · 400 bp

Back to Eriachne

Pi 0.0221

MarkerSeek score 67.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 45
pi: 0.0221
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	469-529 bp	1.0	91.2
trnT-UGU-trnL-UAA_p2	ACGAGGTCCTCTATAACGGG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	58.0	58.0	824-884 bp	1.0	89.7
trnT-UGU-trnL-UAA_p3	ACGAGGTCCTCTATAACGGG	AAAGTGGGATTTGGCTCAGG	55.0	50.0	58.0	58.1	920-980 bp	1.0	88.7
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	477-537 bp	0.956522	88.5
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	AAAGTGGGATTTGGCTCAGG	50.0	50.0	58.0	58.1	573-633 bp	0.956522	87.2