Hotspot

psaA-ycf3

LSC · 41776-42399 · 624 bp

Back to Eriachne

Pi 0.0182

MarkerSeek score 68.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 59
pi: 0.0182
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ACTTCTGATTCCGGTGAACG	GGTTGAAGATTACGAAGCGC	50.0	50.0	57.9	57.6	672-701 bp	1.0	85.7
psaA-ycf3_p2	GTTTTTGAGAAATGCCCGGG	GGTTGAAGATTACGAAGCGC	50.0	50.0	57.9	57.6	747-776 bp	1.0	84.8
psaA-ycf3_p3	TTTTTGAGAAATGCCCGGGT	GGTTGAAGATTACGAAGCGC	45.0	50.0	58.3	57.6	746-775 bp	1.0	83.2
psaA-ycf3_p4	TTTGAGAAATGCCCGGGTTT	GGTTGAAGATTACGAAGCGC	45.0	50.0	58.3	57.6	760 bp	0.043478	35.9
psaA-ycf3_p5	TTTTGAGAAATGCCCGGGTT	GGTTGAAGATTACGAAGCGC	45.0	50.0	58.3	57.6	761 bp	0.043478	35.9