Hotspot

trnC-GCA-rpoB

LSC · 18445-19623 · 1179 bp

Back to Eriachne

Pi 0.0169

MarkerSeek score 64.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 94
pi: 0.0169
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAGACGCGGGAATAGAGAGA	TTGTGGTCTAATTCCGAGCG	50.0	50.0	57.9	58.0	2286-2380 bp	0.913043	86.0
trnC-GCA-rpoB_p2	CAACTTATGGGTAGGGGCAC	TTGTGGTCTAATTCCGAGCG	55.0	50.0	57.9	58.0	1730-1759 bp	0.826087	78.7
trnC-GCA-rpoB_p3	ATTTCTTCGCGAACTCCCTC	TCCCTTTCTTTTATCGCGGG	50.0	50.0	58.0	58.0	1788-1818 bp	0.478261	65.0
trnC-GCA-rpoB_p4	ATTTCTTCGCGAACTCCCTC	TTGTGGTCTAATTCCGAGCG	50.0	50.0	58.0	58.0	2001-2031 bp	0.478261	64.6
trnC-GCA-rpoB_p5	ATTTCTTCGCGAACTCCCTC	GACCCCGAAAATACCAGGTC	50.0	55.0	58.0	58.0	2303-2334 bp	0.478261	63.8