Hotspot

trnG-UCC-trnfM-CAU

LSC · 12467-12967 · 501 bp

Back to Eriachne

Pi 0.0362
MarkerSeek score 73.40
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
501
variable_sites
66
pi
0.0362
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.89
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 GGTAAAACATCCCCTTGCCA ACATAGCGGAGTAGAGCAGT 50.0 50.0 58.1 57.9 532-634 bp 0.913043 86.8
trnG-UCC-trnfM-CAU_p2 TGGTAAAACATCCCCTTGCC ACATAGCGGAGTAGAGCAGT 50.0 50.0 58.1 57.9 533-635 bp 0.913043 86.8
trnG-UCC-trnfM-CAU_p3 GCGAGCGTAGTTCAATGGTA ACATAGCGGAGTAGAGCAGT 50.0 50.0 58.1 57.9 548-650 bp 0.913043 86.5
trnG-UCC-trnfM-CAU_p4 GGTAAAACATCCCCTTGCCA AGCCCTACATAGCGGAGTAG 50.0 55.0 58.1 58.1 538-640 bp 0.869565 84.6
trnG-UCC-trnfM-CAU_p5 TGGTAAAACATCCCCTTGCC AGCCCTACATAGCGGAGTAG 50.0 55.0 58.1 58.1 539-641 bp 0.869565 84.6