Hotspot

psbK-psbI

LSC · 7318-7725 · 408 bp

Back to Eriachne

Pi 0.0242

MarkerSeek score 71.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 50
pi: 0.0242
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	50.0	31.8	57.9	54.4	448-472 bp	1.0	51.9
psbK-psbI_p2	TTAGCGTTTGTTTGGCAAGC	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.2	54.4	457-481 bp	1.0	51.8
psbK-psbI_p3	CGTGGATGTTATGCCTGTCA	ACAAAAAGTTTGAGAGTAAGCA	50.0	31.8	58.0	54.4	497-521 bp	1.0	51.5
psbK-psbI_p4	CGTGGATGTTATGCCTGTCA	AACAAAAAGTTTGAGAGTAAGCA	50.0	30.4	58.0	55.2	498-522 bp	1.0	51.5
psbK-psbI_p5	ACCCGAAGCTTATGCCATTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	57.9	54.4	527-551 bp	1.0	51.3