Hotspot

ndhF

SSC · 111272-113497 · 2226 bp

Back to Eremurus

Pi 0.00546

MarkerSeek score 45.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 60
pi: 0.00546
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGTATCCCACGAAGACCTGT	TGGGTGAATCGGAAACAATT	50.0	40.0	57.8	55.5	2561-2775 bp	1.0	62.9
ndhF_p2	ACAGGAACAAGAGGGATCCA	AATGGGTGAATCGGAAACAA	50.0	40.0	58.0	55.5	2872-2932 bp	0.857143	58.2
ndhF_p3	ACAGGAACAAGAGGGATCCA	TGGGTGAATCGGAAACAATT	50.0	40.0	58.0	55.5	2870-2930 bp	0.857143	58.2
ndhF_p4	AAGAGGGATCCACCGAAGAA	AATGGGTGAATCGGAAACAA	50.0	40.0	58.0	55.5	2864-2924 bp	0.857143	58.2
ndhF_p5	AAGAGGGATCCACCGAAGAA	TGGGTGAATCGGAAACAATT	50.0	40.0	58.0	55.5	2862-2922 bp	0.857143	58.2