Hotspot

ndhE-ndhG

SSC · 118793-118980 · 188 bp

Back to Eremurus

Pi 0.02

MarkerSeek score 60.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 188
variable_sites: 12
pi: 0.02
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TGCTCTAACCATGTTTCGACT	AGGTGCAATTACTATGGCTCG	42.9	47.6	57.3	58.2	305-314 bp	1.0	62.0
ndhE-ndhG_p2	AGTGCTCTAACCATGTTTCGA	AGGTGCAATTACTATGGCTCG	42.9	47.6	57.3	58.2	307-316 bp	1.0	61.9
ndhE-ndhG_p3	GTGCTCTAACCATGTTTCGAC	AGGTGCAATTACTATGGCTCG	47.6	47.6	57.3	58.2	306-315 bp	1.0	61.6
ndhE-ndhG_p4	AGTGCTCTAACCATGTTTCGA	GGTGCAATTACTATGGCTCG	42.9	50.0	57.3	56.7	306-315 bp	1.0	60.6
ndhE-ndhG_p5	TGCTCTAACCATGTTTCGACT	GGTGCAATTACTATGGCTCG	42.9	50.0	57.3	56.7	304-313 bp	1.0	60.6