Hotspot

rpl32-trnL-UAG

SSC · 114374-114874 · 501 bp

Back to Eremurus

Pi 0.0185
MarkerSeek score 52.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
501
variable_sites
38
pi
0.0185
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAATGGTCCATTTTGAGCA AAAAAGGGGGCCCATTACAG 40.0 50.0 55.2 58.0 757-1066 bp 0.666667 48.4
rpl32-trnL-UAG_p2 AGAATGGTCCATTTTGAGCA AGCGTGTCTACCGATTTCAC 40.0 50.0 55.2 58.0 623-931 bp 0.666667 48.3
rpl32-trnL-UAG_p3 AGAATGGTCCATTTTGAGCA CAGCGTGTCTACCGATTTCA 40.0 50.0 55.2 58.0 624-932 bp 0.666667 48.3
rpl32-trnL-UAG_p4 AGAATGGTCCATTTTGAGCA CATCCCCTTTTCCGACGAAT 40.0 50.0 55.2 58.0 893-1202 bp 0.666667 48.0
rpl32-trnL-UAG_p5 AGAATGGTCCATTTTGAGCA TGGCGAAACCTTGGGTAAAA 40.0 45.0 55.2 57.9 1098-1407 bp 0.666667 47.4