Hotspot

trnL-UAA-trnF-GAA

LSC · 47440-47914 · 475 bp

Back to Eremurus

Pi 0.0159
MarkerSeek score 58.80
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
475
variable_sites
34
pi
0.0159
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.96
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 513-590 bp 1.0 80.8
trnL-UAA-trnF-GAA_p2 GTTCAAGTCCCTCTATCCCC TTTGAACTGGTGACACGAGG 55.0 50.0 56.7 58.1 514-591 bp 1.0 78.1
trnL-UAA-trnF-GAA_p3 GGGTTCAAGTCCCTCTATCC TTTGAACTGGTGACACGAGG 55.0 50.0 56.7 58.1 516-593 bp 1.0 78.0
trnL-UAA-trnF-GAA_p4 GGTTCAAGTCCCTCTATCCC TTTGAACTGGTGACACGAGG 55.0 50.0 56.7 58.1 515-592 bp 1.0 78.0
trnL-UAA-trnF-GAA_p5 TTCAAGTCCCTCTATCCCCA TACCAACTGAGCTATCCCGA 50.0 50.0 57.0 57.6 475-552 bp 1.0 78.0