Hotspot

psbM-trnD-GUC

LSC · 29536-30536 · 1001 bp

Back to Eremurus

Pi 0.00715
MarkerSeek score 59.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1001
variable_sites
32
pi
0.00715
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTGGCTGACTGTTTTTACGT ATGTATGCTATACGCGCCTC 40.0 50.0 55.8 57.7 1189-1204 bp 1.0 63.6
psbM-trnD-GUC_p2 TGGCTGACTGTTTTTACGTAGA ATGTATGCTATACGCGCCTC 40.9 50.0 57.7 57.7 1188-1203 bp 1.0 62.4
psbM-trnD-GUC_p3 TTGGCTGACTGTTTTTACGT TTCAATTGGTCAGAGCACCG 40.0 50.0 55.8 58.5 1156-1171 bp 1.0 62.0
psbM-trnD-GUC_p4 TTTGGCTGACTGTTTTTACGT ATGTATGCTATACGCGCCTC 38.1 50.0 56.6 57.7 1190-1205 bp 1.0 61.8
psbM-trnD-GUC_p5 AGTGCGAGAATATTGACTTCCA ATGTATGCTATACGCGCCTC 40.9 50.0 57.5 57.7 1116-1131 bp 1.0 61.5