Hotspot

trnS-GCU-trnG-UCC

LSC · 7918-8802 · 885 bp

Back to Eremurus

Pi 0.0119

MarkerSeek score 67.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 47
pi: 0.0119
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	968-1031 bp	1.0	72.6
trnS-GCU-trnG-UCC_p2	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	969-1032 bp	1.0	72.6
trnS-GCU-trnG-UCC_p3	AGATTAGCAATCTGCCGCTT	AACCGAAAGACCCCTTAACT	45.0	45.0	57.7	56.4	976-1039 bp	1.0	71.9
trnS-GCU-trnG-UCC_p4	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1022-1085 bp	1.0	71.7
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	960-1023 bp	1.0	71.4