Hotspot

rps16-trnQ-UUG

LSC · 5618-6628 · 1011 bp

Back to Eremurus

Pi 0.00642
MarkerSeek score 55.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1011
variable_sites
29
pi
0.00642
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCGAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 809-1111 bp 1.0 85.4
rps16-trnQ-UUG_p2 ATCCACAGCGAATCATGTCC GTCACTCGGAGGTTCGAATC 50.0 55.0 58.0 58.1 817-1119 bp 1.0 85.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 786-1088 bp 1.0 84.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTCACTCGGAGGTTCGAATC 45.0 55.0 58.1 58.1 794-1096 bp 1.0 84.5
rps16-trnQ-UUG_p5 ATCCACAGCGAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 813-1115 bp 1.0 84.3