Hotspot

psaC-ndhE

SSC · 108579-109048 · 470 bp

Back to Eremopyrum

Pi 0.00883

MarkerSeek score 64.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 470
variable_sites: 4
pi: 0.00883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	648-665 bp	1.0	80.2
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	649-666 bp	1.0	80.2
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	609-626 bp	1.0	79.3
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	610-627 bp	1.0	79.3
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	50.0	50.0	57.9	58.3	611-628 bp	1.0	79.3