Hotspot

rpl32-trnL-UAG

SSC · 104726-105424 · 699 bp

Back to Eremopyrum

Pi 0.0078

MarkerSeek score 54.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 699
variable_sites: 5
pi: 0.0078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGGCAACAAACAAATAAAAAGA	TTCGAACCGAGATGCTTGAG	30.4	50.0	56.0	58.0	741-799 bp	1.0	44.6
rpl32-trnL-UAG_p2	TGGGCAACAAACAAATAAAAAGA	GGATTCGAACCGAGATGCTT	30.4	50.0	56.0	58.1	744-802 bp	1.0	44.5
rpl32-trnL-UAG_p3	TGGGCAACAAACAAATAAAAAGA	TCCTAAGAGCAGCGTGTCTA	30.4	50.0	56.0	57.9	714-772 bp	1.0	44.5
rpl32-trnL-UAG_p4	TGGGCAACAAACAAATAAAAAGA	TTTTTCAAGAATGCCGCCAC	30.4	45.0	56.0	57.9	766-824 bp	1.0	44.5
rpl32-trnL-UAG_p5	TGGGCAACAAACAAATAAAAAGA	AGCAGCGTGTCTACCAATTT	30.4	45.0	56.0	57.8	707-765 bp	1.0	44.5