Hotspot

ndhF-rpl32

SSC · 103625-104533 · 909 bp

Back to Eremopyrum

Pi 0.0119
MarkerSeek score 66.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
909
variable_sites
10
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 955-1020 bp 1.0 44.8
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 928-993 bp 1.0 44.8
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 929-994 bp 1.0 44.8
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 930-995 bp 1.0 44.8
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 931-996 bp 1.0 44.8