Hotspot

ndhC-trnV-UAC

LSC · 50073-50977 · 905 bp

Back to Eremopyrum

Pi 0.00562

MarkerSeek score 63.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 5
pi: 0.00562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCGAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.1	57.9	1000-1015 bp	1.0	80.4
ndhC-trnV-UAC_p2	GCCCTGAAATCGAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.1	58.3	1001-1016 bp	1.0	78.6
ndhC-trnV-UAC_p3	CTCTGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.3	57.9	1029-1044 bp	1.0	75.7
ndhC-trnV-UAC_p4	TCTGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.7	57.9	1028-1043 bp	1.0	75.0
ndhC-trnV-UAC_p5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.2	57.9	1019-1034 bp	1.0	74.8