Hotspot

trnF-GAA-ndhJ

LSC · 47804-48400 · 597 bp

Back to Eremopyrum

Pi 0.00531

MarkerSeek score 63.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 3
pi: 0.00531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	50.0	50.0	58.1	57.9	727-732 bp	1.0	82.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AAAGTTGGATAGGTTGGCCC	50.0	50.0	58.1	57.8	700-705 bp	1.0	81.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	50.0	45.0	58.1	57.5	739-744 bp	1.0	79.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	50.0	50.0	58.1	58.5	730-735 bp	1.0	79.4
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	CGCGCCTTAAACGTATCCTA	50.0	50.0	57.5	57.9	730-735 bp	1.0	79.2