Hotspot

rpoB-rpoC1

LSC · 22914-22950 · 37 bp

Back to Eremopyrum

Pi 0.0714

MarkerSeek score 70.60

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.38
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	GCTCGTTCGAGAACTACGAT	CGCCAACGACTTCTCCATTA	50.0	50.0	57.5	58.0	211-234 bp	1.0	80.7
rpoB-rpoC1_p2	GCTCGTTCGAGAACTACGAT	GGTTTTTGTTAGCCCAAGCC	50.0	50.0	57.5	57.8	187-210 bp	1.0	79.5
rpoB-rpoC1_p3	AGTGCTCGTTCGAGAACTAC	CGCCAACGACTTCTCCATTA	50.0	50.0	57.4	58.0	214-237 bp	1.0	79.4
rpoB-rpoC1_p4	GCTCGTTCGAGAACTACGAT	TTGTTGAGGGGAAACGAGTC	50.0	50.0	57.5	57.8	162-185 bp	1.0	78.8
rpoB-rpoC1_p5	GTGCTCGTTCGAGAACTACG	CGCCAACGACTTCTCCATTA	55.0	50.0	58.8	58.0	213-236 bp	1.0	78.3