Hotspot

rps16-trnQ-UUG

LSC · 5603-6352 · 750 bp

Back to Eremopyrum

Pi 0.0107

MarkerSeek score 65.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 8
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	820-824 bp	1.0	79.4
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	831-835 bp	1.0	78.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	45.0	45.0	58.1	57.8	889-893 bp	1.0	78.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	824-828 bp	1.0	78.4
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.2	57.8	900-904 bp	1.0	78.0