Hotspot

rpl16

LSC · 78765-80261 · 1497 bp

Back to Eremopogon

Pi 0.00468

MarkerSeek score 53.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 7
pi: 0.00468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCAGGGG	AGAAGAAAGTATCCCTCTTTC	47.6	38.1	58.0	52.3	1614-1628 bp	1.0	40.4
rpl16_p2	TCCAGAAAGAAAAACCAGGGG	AAGAAGAAAGTATCCCTCTTTC	47.6	36.4	58.0	53.2	1615-1629 bp	1.0	40.4
rpl16_p3	TCCAGAAAGAAAAACCAGGGG	AAAGAAGAAAGTATCCCTCTTTC	47.6	34.8	58.0	54.0	1616-1630 bp	1.0	40.4
rpl16_p4	CCAGAAAGAAAAACCAGGGGT	AGAAGAAAGTATCCCTCTTTC	47.6	38.1	58.3	52.3	1613-1627 bp	1.0	40.4
rpl16_p5	CCAGAAAGAAAAACCAGGGGT	AAGAAGAAAGTATCCCTCTTTC	47.6	36.4	58.3	53.2	1614-1628 bp	1.0	40.4