Hotspot

ndhF-rpl32

SSC · 106606-107460 · 855 bp

Back to Eremopogon

Pi 0.0117
MarkerSeek score 65.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
855
variable_sites
10
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAGAGGA TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 968-988 bp 1.0 42.5
ndhF-rpl32_p2 CTGGAAGTGGGAGAAGAGGA TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 940-960 bp 1.0 42.3
ndhF-rpl32_p3 CTGGAAGTGGGAGAAGAGGA TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 941-961 bp 1.0 42.3
ndhF-rpl32_p4 CTGGAAGTGGGAGAAGAGGA TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 942-962 bp 1.0 42.3
ndhF-rpl32_p5 CTGGAAGTGGGAGAAGAGGA TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 943-963 bp 1.0 42.3