Hotspot

trnP-UGG-psaJ

LSC · 65416-65795 · 380 bp

Back to Eremopogon

Pi 0.0132

MarkerSeek score 61.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCCCCACTACTGCCCATAAA	AAACCTGCTAAAGCCCCAAA	50.0	45.0	58.0	57.9	567 bp	1.0	79.7
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	490 bp	1.0	79.1
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	491 bp	1.0	78.7
trnP-UGG-psaJ_p4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	498 bp	1.0	78.5
trnP-UGG-psaJ_p5	CCCCACTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	55.0	45.0	57.7	57.9	566 bp	1.0	77.2