Hotspot

petA-psbJ

LSC · 61690-62593 · 904 bp

Back to Eremopogon

Pi 0.0111

MarkerSeek score 56.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 10
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	1040-1041 bp	1.0	71.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	1038-1039 bp	1.0	70.2
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	50.0	50.0	57.9	56.3	1039-1040 bp	1.0	67.0
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	1047-1048 bp	1.0	66.6
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGGG	50.0	52.4	57.9	58.8	1039-1040 bp	1.0	66.1