Hotspot

rpoC2-rps2

LSC · 30686-30999 · 314 bp

Back to Eremopogon

Pi 0.0159

MarkerSeek score 61.90

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 314
variable_sites: 5
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	GAGACCCCGTTTACCCCTAT	ACCAAAATGAACTCCCGCTT	55.0	45.0	58.2	58.0	410 bp	1.0	79.3
rpoC2-rps2_p2	AGACCCCGTTTACCCCTATT	ACCAAAATGAACTCCCGCTT	50.0	45.0	57.7	58.0	409 bp	1.0	78.7
rpoC2-rps2_p3	CATCAGAGACCCCGTTTACC	ACCAAAATGAACTCCCGCTT	55.0	45.0	57.7	58.0	415 bp	1.0	78.4
rpoC2-rps2_p4	GAGACCCCGTTTACCCCTAT	TTTACGCTTTGCCGAGATGT	55.0	45.0	58.2	58.2	467 bp	1.0	77.7
rpoC2-rps2_p5	GAGACCCCGTTTACCCCTAT	GGGGACATTTTGGGATTCCA	55.0	50.0	58.2	57.8	445 bp	1.0	77.3