Hotspot

trnE-UUC-trnY-GUA

LSC · 16425-16485 · 61 bp

Back to Eremopogon

Pi 0.0164

MarkerSeek score 61.90

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 1
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	ACGGTGCTATCGTCTAGTGA	CGAATTTACAGTCCGTCCCC	50.0	55.0	58.0	58.4	198 bp	1.0	77.3
trnE-UUC-trnY-GUA_p2	ACGGTGCTATCGTCTAGTGA	ACGAATTTACAGTCCGTCCC	50.0	50.0	58.0	57.6	199 bp	1.0	77.1
trnE-UUC-trnY-GUA_p3	CTCTCTTTCAAGGAGGCAGC	CGAATTTACAGTCCGTCCCC	55.0	55.0	58.3	58.4	149 bp	1.0	75.6
trnE-UUC-trnY-GUA_p4	ACGGTGCTATCGTCTAGTGA	CAAATTTTTGGGCCGAGCTG	50.0	50.0	58.0	58.6	247 bp	1.0	75.5
trnE-UUC-trnY-GUA_p5	TCGTCTAGTGGTTCAGGACA	CGAATTTACAGTCCGTCCCC	50.0	55.0	57.7	58.4	170 bp	1.0	75.4