Hotspot

trnT-GGU-trnE-UUC

LSC · 15882-16351 · 470 bp

Back to Eremopogon

Pi 0.0527
MarkerSeek score 79.60
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
470
variable_sites
24
pi
0.0527
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GACAGAAGTCATCGGTTCGA TGTCCTGAACCACTAGACGA 50.0 50.0 57.4 57.7 526-528 bp 1.0 78.6
trnT-GGU-trnE-UUC_p2 GACAGAAGTCATCGGTTCGA GCTGCCTCCTTGAAAGAGAG 50.0 55.0 57.4 58.3 547-549 bp 1.0 78.5
trnT-GGU-trnE-UUC_p3 GACAGAAGTCATCGGTTCGA CCTGAACCACTAGACGATGG 50.0 55.0 57.4 57.4 523-525 bp 1.0 76.2
trnT-GGU-trnE-UUC_p4 GACAGAAGTCATCGGTTCGA CTGAACCACTAGACGATGGG 50.0 55.0 57.4 57.4 522-524 bp 1.0 76.2
trnT-GGU-trnE-UUC_p5 GACAGAAGTCATCGGTTCGAA GCTGCCTCCTTGAAAGAGAG 47.6 55.0 58.1 58.3 547-549 bp 1.0 75.1