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trnK-UUU-rps16

LSC · 3993-4565 · 573 bp

Back to Eremopogon

Pi 0.00545

MarkerSeek score 61.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 3
pi: 0.00545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAACA	50.0	47.6	58.3	58.2	689-710 bp	1.0	71.5
trnK-UUU-rps16_p2	CTTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAAGAACA	50.0	47.6	57.5	58.2	694-715 bp	1.0	69.4
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAAC	50.0	50.0	58.3	56.4	689-710 bp	1.0	67.6
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGTGCTCAACCTACAAGAACA	50.0	47.6	57.2	58.2	688-709 bp	1.0	67.5
trnK-UUU-rps16_p5	TCTACCAAGAGTCTGGACGA	GGTGCTCAACCTACAAGAACA	50.0	47.6	57.1	58.2	745-766 bp	1.0	66.5