primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	58.2	58.7	20.3	0.0	1.837155	4290	5278	97-1997	0.984375	76.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	58.2	58.7	20.3	0.0	1.837155	4290	5278	97-1997	0.984375	76.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	58.3	58.7	0.0	0.0	1.934573	4290	5278	896-1996	0.994792	76.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	TGCTCAACCCACAGGAACT	20	19	50.0	52.6	58.2	58.8	20.3	0.0	1.937756	4290	5278	100-1996	0.984375	75.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TGCTCAACCCACAGGAACT	20	19	50.0	52.6	58.2	58.8	20.3	0.0	1.937756	4290	5278	100-1996	0.984375	75.9
atpH-atpI_p1	atpH-atpI	1	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.1	58.5	0.0	0.0	0.655741	14147	15171	1002-1306	1.000000	86.8
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.1	58.5	0.0	0.0	0.621503	14147	15171	917-1221	0.994792	86.8
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.9	58.5	0.0	0.0	0.666168	14147	15171	935-1239	0.994792	86.5
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.732109	14147	15171	934-1238	0.994792	85.9
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	58.3	58.5	0.0	0.0	0.779884	14147	15171	929-1233	1.000000	85.8
rps2-rpoC2_p1	rps2-rpoC2	1	TGCAGAGATATAGGGTGCCA	TCGATGATACATCAGAACAATCA	20	23	50.0	34.8	57.9	55.6	0.0	11.4	5.514818	16852	17080	106-2120	0.958333	50.3
rps2-rpoC2_p2	rps2-rpoC2	2	TGCAGAGATATAGGGTGCCA	TTCGATGATACATCAGAACAATCA	20	24	50.0	33.3	57.9	56.3	0.0	11.4	5.821174	16852	17080	107-2121	0.958333	50.3
rps2-rpoC2_p3	rps2-rpoC2	3	TGCAGAGATATAGGGTGCCA	CGATGATACATCAGAACAATCA	20	22	50.0	36.4	57.9	54.1	0.0	11.4	5.977913	16852	17080	103-768	0.958333	50.3
rps2-rpoC2_p4	rps2-rpoC2	4	CCTCCAGCATCTCTTCCAAG	TCGATGATACATCAGAACAATCA	20	23	55.0	34.8	57.7	55.6	0.0	11.4	5.741997	16852	17080	80-2122	0.947917	49.9
rps2-rpoC2_p5	rps2-rpoC2	5	AAATGAACTCCTGCCTCCAG	TCGATGATACATCAGAACAATCA	20	23	50.0	34.8	57.5	55.6	0.0	11.4	5.917073	16852	17080	80-2119	0.906250	48.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28737	29578	80-993	1.000000	91.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28737	29578	80-991	1.000000	91.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162414	28737	29578	80-994	1.000000	90.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	TCTAGAGCCCACTTCTTCCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.214765	28737	29578	80-1003	0.994792	90.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28737	29578	80-939	0.994792	89.9
petN-psbM_p1	petN-psbM	1	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCGCTATTCATTCT	20	21	50.0	42.9	58.0	57.5	0.0	9.7	1.464603	29669	30652	80-1123	1.000000	80.3
petN-psbM_p2	petN-psbM	2	ACTCGTAGTGTGGGGAAGAA	TGCTACTGCGCTATTCATTCT	20	21	50.0	42.9	58.0	57.5	0.0	9.7	1.464603	29669	30652	80-1125	0.994792	80.1
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCGCTATTCATTCT	20	21	55.0	42.9	57.9	57.5	0.0	9.7	1.539518	29669	30652	80-1122	1.000000	79.7
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTATTCATTCT	20	21	50.0	42.9	58.2	57.5	0.0	9.7	1.610581	29669	30652	80-1177	1.000000	79.1
petN-psbM_p5	petN-psbM	5	GTAGTGTGGGGAAGAAGTGG	TGCTACTGCGCTATTCATTCT	20	21	55.0	42.9	57.5	57.5	0.0	9.7	1.933642	29669	30652	80-1121	1.000000	76.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	45291	46144	80-1070	0.994792	84.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	45291	46144	80-1069	0.994792	84.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.018457	45291	46144	80-1091	1.000000	83.8
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.020450	45291	46144	80-1090	1.000000	83.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	45291	46144	80-1041	0.994792	83.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	49296	49934	80-818	0.932292	87.9
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	49296	49934	80-782	0.932292	87.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	TGATAATCATCCGCGCTTGA	20	20	55.0	45.0	58.0	57.5	10.3	5.6	0.527074	49296	49934	80-858	1.000000	86.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.223436	49296	49934	80-806	0.932292	86.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GAATGGTCGGGATAGCTCAG	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.534382	49296	49934	80-826	0.932292	84.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.7	0.0	0.279423	51611	52793	80-1349	1.000000	89.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.7	0.0	0.623195	51611	52793	80-1348	1.000000	87.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.605515	51611	52793	80-1370	0.989583	86.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.7	0.0	0.765489	51611	52793	80-1337	1.000000	85.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.6	57.8	0.0	0.0	0.766291	51611	52793	80-1362	1.000000	85.9
atpB-rbcL_p1	atpB-rbcL	1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	55811	56589	80-890	0.994792	87.3
atpB-rbcL_p2	atpB-rbcL	2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.422541	55811	56589	80-931	0.989583	86.7
atpB-rbcL_p3	atpB-rbcL	3	CGGCGGAAAAGCTACATCTA	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.520447	55811	56589	80-962	1.000000	86.6
atpB-rbcL_p4	atpB-rbcL	4	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.541987	55811	56589	80-937	1.000000	86.3
atpB-rbcL_p5	atpB-rbcL	5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.659357	55811	56589	80-891	0.994792	85.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	66015	66936	80-1137	1.000000	84.1
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	1.030402	66015	66936	80-1150	1.000000	83.8
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	66015	66936	80-1060	1.000000	83.2
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	66015	66936	80-1146	1.000000	83.1
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	66015	66936	80-1145	1.000000	83.0
petD_p1	petD	1	GGAGTTGAAGGGAATTCTCCG	CTTTGAAGCGCCTATTCCCT	21	20	52.4	50.0	58.4	58.0	0.4	2.3	1.410635	77560	78786	80-1344	0.994792	80.5
petD_p2	petD	2	GGAGTTGAAGGGAATTCTCCG	TGAAGCGCCTATTCCCTAGA	21	20	52.4	50.0	58.4	57.9	0.4	2.3	1.448652	77560	78786	80-1341	0.994792	80.2
petD_p3	petD	3	GAGTTGAAGGGAATTCTCCGA	CTTTGAAGCGCCTATTCCCT	21	20	47.6	50.0	57.4	58.0	0.4	2.3	1.655888	77560	78786	80-1343	0.994792	78.5
petD_p4	petD	4	GAGTTGAAGGGAATTCTCCGA	TGAAGCGCCTATTCCCTAGA	21	20	47.6	50.0	57.4	57.9	0.4	2.3	1.693906	77560	78786	80-1340	0.994792	78.2
petD_p5	petD	5	TGGAGTTGAAGGGAATTCTCC	CTTTGAAGCGCCTATTCCCT	21	20	47.6	50.0	57.3	58.0	0.4	2.3	1.765210	77560	78786	80-1345	0.994792	77.7
ycf2_p1	ycf2	1	AATCGGTTGGGTCTTCCA	CCAGCGACGGTTTCATTAGA	18	20	50.0	50.0	55.7	58.0	0.0	0.0	4.343868	87748	87867	80-599	0.989583	56.8
ycf2_p2	ycf2	2	AATCGGTTGGGTCTTCCA	TCCAGCGACGGTTTCATTAG	18	20	50.0	50.0	55.7	58.0	0.0	0.0	4.343868	87748	87867	80-600	0.984375	56.6
ycf2_p3	ycf2	3	AATCGGTTGGGTCTTCCA	AATTCCAGCGACGGTTTCAT	18	20	50.0	45.0	55.7	58.2	0.0	0.0	4.518069	87748	87867	80-600	0.984375	55.2
ycf2_p4	ycf2	4	AATCGGTTGGGTCTTCCA	ATTCCAGCGACGGTTTCATT	18	20	50.0	45.0	55.7	58.2	0.0	0.0	4.518069	87748	87867	80-600	0.984375	55.2
ycf2_p5	ycf2	5	AATCGGTTGGGTCTTCCA	AGATCTCAATTCCAGCGACG	18	20	50.0	50.0	55.7	57.8	0.0	0.0	4.555193	87748	87867	80-600	0.989583	55.1
ndhF_p1	ndhF	1	ATTTAGGCAGAACACCGTCC	TTGCGCTTTGCCAATTCATT	20	20	50.0	40.0	57.9	57.8	0.0	9.7	0.276246	110900	113131	80-2989	0.796875	81.7
ndhF_p2	ndhF	2	ATTTAGGCAGAACACCGTCC	TTTGCGCTTTGCCAATTCAT	20	20	50.0	40.0	57.9	57.8	0.0	9.7	0.276246	110900	113131	80-2990	0.796875	81.7
ndhF_p3	ndhF	3	ACACCGTCCCCCATTTTTAC	ATTTGCGCTTTGCCAATTCA	20	20	50.0	40.0	58.1	57.8	0.0	9.7	0.253043	110900	113131	80-2980	0.770833	80.8
ndhF_p4	ndhF	4	ACACCGTCCCCCATTTTTAC	TTGCGCTTTGCCAATTCATT	20	20	50.0	40.0	58.1	57.8	0.0	9.7	0.253043	110900	113131	80-2978	0.770833	80.8
ndhF_p5	ndhF	5	ACACCGTCCCCCATTTTTAC	TTTGCGCTTTGCCAATTCAT	20	20	50.0	40.0	58.1	57.8	0.0	9.7	0.253043	110900	113131	80-2979	0.770833	80.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ATGAATTGGCAAAGCGCAAA	TTTAGGAGATGACATGCCGC	20	20	40.0	50.0	57.8	58.0	11.5	0.0	0.213262	113849	114725	80-1268	0.942708	88.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGAATTGGCAAAGCGCAAAT	TTTAGGAGATGACATGCCGC	20	20	40.0	50.0	57.8	58.0	11.5	0.0	0.213262	113849	114725	80-1267	0.942708	88.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ATGAATTGGCAAAGCGCAAA	CCCCTTTTCGGATGAATCGT	20	20	40.0	50.0	57.8	58.0	11.5	0.0	0.205208	113849	114725	80-2536	0.052083	51.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGAATTGGCAAAGCGCAAAT	CCCCTTTTCGGATGAATCGT	20	20	40.0	50.0	57.8	58.0	11.5	0.0	0.205208	113849	114725	80-2535	0.052083	51.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AATGAATTGGCAAAGCGCAA	CCCCTTTTCGGATGAATCGT	20	20	40.0	50.0	57.8	58.0	11.5	0.0	0.205208	113849	114725	80-2537	0.052083	50.8
ycf1_p1	ycf1	1	ATTTTTCCAGTGTAGGGGCG	ACACCGTCCCCCATTTTTAC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.252034	128943	129062	80-2762	0.203125	52.8
ycf1_p2	ycf1	2	ATTTTTCCAGTGTAGGGGCG	ATTTAGGCAGAACACCGTCC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.275237	128943	129062	80-2773	0.203125	52.5
ycf1_p3	ycf1	3	ATTTTTCCAGTGTAGGGGCG	AGGGTGGAAAGTGAGGAAGA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.284215	128943	129062	80-2717	0.203125	52.4
ycf1_p4	ycf1	4	ATTTTTCCAGTGTAGGGGCG	AGGGCCCTCTTATCTCTTCC	20	20	50.0	55.0	58.2	57.9	0.0	22.3	0.276565	128943	129062	80-1088	0.197917	49.4
ycf1_p5	ycf1	5	ATTTTTCCAGTGTAGGGGCG	GGGCCCTCTTATCTCTTCCT	20	20	50.0	55.0	58.2	57.9	0.0	17.0	0.276565	128943	129062	80-1087	0.197917	49.4