Hotspot

ycf1

SSC · 108195-113627 · 5433 bp

Back to Eremogone

Pi 0.00387

MarkerSeek score 57.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5433
variable_sites: 48
pi: 0.00387
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACATGTTCTGTTCGGCTTAAGA	TCGATTCAAGAATCCGAAAAAC	40.9	36.4	58.0	55.4	166 bp	1.0	42.4
ycf1_p2	ACATGTTCTGTTCGGCTTAAGA	TTCGATTCAAGAATCCGAAAAAC	40.9	34.8	58.0	56.1	167 bp	1.0	42.4
ycf1_p3	ACATGTTCTGTTCGGCTTAAG	TCGATTCAAGAATCCGAAAAAC	42.9	36.4	56.6	55.4	166 bp	1.0	42.4
ycf1_p4	ACATGTTCTGTTCGGCTTAAGA	TCGATTCAAGAATCCGAAAA	40.9	35.0	58.0	53.0	166 bp	1.0	42.4
ycf1_p5	ACATGTTCTGTTCGGCTTAAGA	TTCGATTCAAGAATCCGAAA	40.9	35.0	58.0	53.0	167 bp	1.0	42.4