Hotspot

ndhD-psaC

SSC · 101864-101950 · 87 bp

Back to Eremogone

Pi 0.0368

MarkerSeek score 70.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 87
variable_sites: 6
pi: 0.0368
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCAAGGAAAAGAATTCGTGGT	CAACTCGGAGCATGGGTTTA	42.9	50.0	56.7	57.9	170-175 bp	1.0	63.7
ndhD-psaC_p2	ACCAAGGAAAAGAATTCGTGG	CAACTCGGAGCATGGGTTTA	42.9	50.0	56.7	57.9	171-176 bp	1.0	63.6
ndhD-psaC_p3	ACCAAGGAAAAGAATTCGTGG	GAACGGAAGACTGTGTTGGT	42.9	50.0	56.7	58.1	258-263 bp	1.0	63.2
ndhD-psaC_p4	CCAAGGAAAAGAATTCGTGGT	GAACGGAAGACTGTGTTGGT	42.9	50.0	56.7	58.1	257-262 bp	1.0	63.2
ndhD-psaC_p5	ACCAAGGAAAAGAATTCGTGGT	GAACGGAAGACTGTGTTGGT	40.9	50.0	58.4	58.1	258-263 bp	1.0	61.9