Hotspot

ndhF-rpl32

SSC · 98062-98469 · 408 bp

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Pi 0.0221
MarkerSeek score 61.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
408
variable_sites
14
pi
0.0221
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGAAGGGTATGATCCATGCA ACGAATACGCTTTTTCGAAA 45.0 35.0 56.3 54.3 496-539 bp 1.0 43.7
ndhF-rpl32_p2 TTGGAAGTGGAATGAAGGGT ACGAATACGCTTTTTCGAAA 45.0 35.0 56.6 54.3 508-551 bp 1.0 43.6
ndhF-rpl32_p3 CCCACTCCGATTAACAAAGGA ACGAATACGCTTTTTCGAAA 47.6 35.0 57.7 54.3 530-573 bp 1.0 43.5
ndhF-rpl32_p4 TCCCACTCCGATTAACAAAGG ACGAATACGCTTTTTCGAAA 47.6 35.0 57.7 54.3 531-574 bp 1.0 43.5
ndhF-rpl32_p5 CCCACTCCGATTAACAAAGGA ACGAATACGCTTTTTCGAAAT 47.6 33.3 57.7 54.9 530-573 bp 1.0 43.5