Hotspot

psaJ-rpl33

LSC · 58245-58623 · 379 bp

Back to Eremogone

Pi 0.0267

MarkerSeek score 67.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 23
pi: 0.0267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AGATGCATTGACATTCCCCT	AAAACGCCCCTTGATTCCTT	45.0	45.0	56.9	58.0	505-548 bp	1.0	77.5
psaJ-rpl33_p2	CAGATGCATTGACATTCCCC	AAAACGCCCCTTGATTCCTT	50.0	45.0	56.8	58.0	506-549 bp	1.0	77.2
psaJ-rpl33_p3	CCAGATGCATTGACATTCCC	AAAACGCCCCTTGATTCCTT	50.0	45.0	56.8	58.0	507-550 bp	1.0	77.2
psaJ-rpl33_p4	CCCAGATGCATTGACATTCC	AAAACGCCCCTTGATTCCTT	50.0	45.0	56.8	58.0	508-551 bp	1.0	77.2
psaJ-rpl33_p5	CAGATGCATTGACATTCCCCT	AAAACGCCCCTTGATTCCTT	47.6	45.0	58.3	58.0	506-549 bp	1.0	75.9