Hotspot

petA-psbJ

LSC · 55112-55752 · 641 bp

Back to Eremogone

Pi 0.0112

MarkerSeek score 54.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 16
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTGGGTGGATTTGGTCAAG	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1290-1302 bp	1.0	82.0
petA-psbJ_p2	TGTGGGTGGATTTGGTCAAG	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1288-1300 bp	1.0	82.0
petA-psbJ_p3	TGTGGGTGGATTTGGTCAAG	TGATGAAATGCGGAGGGAAG	50.0	50.0	58.0	58.0	955-971 bp	1.0	81.9
petA-psbJ_p4	TGTGGGTGGATTTGGTCAAG	CTTGATGAAATGCGGAGGGA	50.0	50.0	58.0	58.0	957-973 bp	1.0	81.8
petA-psbJ_p5	TGTGGGTGGATTTGGTCAAG	GATGAAATGCGGAGGGAAGT	50.0	50.0	58.0	58.0	954-970 bp	1.0	81.8