Hotspot

atpB-rbcL

LSC · 47468-48194 · 727 bp

Back to Eremogone

Pi 0.00523

MarkerSeek score 63.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 9
pi: 0.00523
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTTGAGCGATACGTCCCAG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.8	56.6	820-838 bp	1.0	68.8
atpB-rbcL_p2	GCCTCGAGAAAAAGCTACGT	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.3	56.6	857-875 bp	1.0	68.2
atpB-rbcL_p3	TGCCTCGAGAAAAAGCTACG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.3	56.6	858-876 bp	1.0	68.2
atpB-rbcL_p4	GCGATACGTCCCAGGTTTTT	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.6	56.6	814-832 bp	1.0	66.2
atpB-rbcL_p5	AATTTGAGCGATACGTCCCA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.3	56.6	821-839 bp	1.0	65.1