Hotspot

ndhC-trnV

LSC · 43881-44546 · 666 bp

Back to Eremogone

Pi 0.00661

MarkerSeek score 63.30

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 10
pi: 0.00661
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TTTTCCGGCCCTTTGCTAAT	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.1	57.8	777-826 bp	1.0	82.9
ndhC-trnV_p2	TTTTCCGGCCCTTTGCTAAT	ACTGCATGTTGGGTCTTTGA	45.0	45.0	58.1	57.6	849-898 bp	1.0	81.5
ndhC-trnV_p3	TTTTTCCGGCCCTTTGCTAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.3	57.8	778-827 bp	1.0	81.1
ndhC-trnV_p4	GCTAATCGGCGCTAAAATGC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	763-812 bp	1.0	80.8
ndhC-trnV_p5	GGCGCTAAAATGCTGGAAAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.6	57.8	756-805 bp	1.0	80.5