Hotspot

psaA-ycf3

LSC · 36299-36996 · 698 bp

Back to Eremogone

Pi 0.00587

MarkerSeek score 64.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 9
pi: 0.00587
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	746-902 bp	1.0	83.9
psaA-ycf3_p2	GTTCTTGAAAAATGGCCGGG	TTGGTTGAAGATCACGGGAC	50.0	50.0	57.9	57.8	679-835 bp	1.0	82.8
psaA-ycf3_p3	ACGACGTTTTTACGGGATCC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.3	58.0	709-865 bp	1.0	82.3
psaA-ycf3_p4	TTCTTGAAAAATGGCCGGGT	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.3	58.0	745-901 bp	1.0	82.2
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	742-898 bp	1.0	82.1