Hotspot

psbZ-trnG-GCC

LSC · 30710-30993 · 284 bp

Back to Eremogone

Pi 0.0626

MarkerSeek score 64.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 44
pi: 0.0626
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TACTTCTCCCGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	440 bp	1.0	86.8
psbZ-trnG-GCC_p2	TCCCGATGGTTGGTCAAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	434 bp	1.0	84.4
psbZ-trnG-GCC_p3	CCCGATGGTTGGTCAAGTAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.5	57.8	433 bp	1.0	83.3
psbZ-trnG-GCC_p4	TTCTCCCGATGGTTGGTCAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	437 bp	1.0	79.7
psbZ-trnG-GCC_p5	TTACTTCTCCCGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.9	57.8	441 bp	1.0	78.6