Hotspot

psbM-trnD-GUC

LSC · 25789-26284 · 496 bp

Back to Eremogone

Pi 0.00901

MarkerSeek score 61.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 10
pi: 0.00901
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGCGCAGTAGC	TCAATTGGTTAGAGCACCGC	50.0	50.0	58.1	58.6	552-615 bp	1.0	80.4
psbM-trnD-GUC_p2	AATGAAGAGCGCAGTAGCAA	TCAATTGGTTAGAGCACCGC	45.0	50.0	57.9	58.6	550-613 bp	1.0	80.1
psbM-trnD-GUC_p3	ATGAAGAGCGCAGTAGCAAT	TCAATTGGTTAGAGCACCGC	45.0	50.0	57.7	58.6	549-612 bp	1.0	78.4
psbM-trnD-GUC_p4	AGAATGAAGAGCGCAGTAGC	AGCACTTAAGTTGCTTGGGA	50.0	45.0	58.1	57.3	633 bp	0.2	42.2
psbM-trnD-GUC_p5	AATGAAGAGCGCAGTAGCAA	AGCACTTAAGTTGCTTGGGA	45.0	45.0	57.9	57.3	631 bp	0.2	42.0