Hotspot

petN-psbM

LSC · 25463-25683 · 221 bp

Back to Eremogone

Pi 0.0201

MarkerSeek score 70.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 11
pi: 0.0201
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	GCTACTGCGCTCTTCATTCT	50.0	50.0	57.6	58.1	321-474 bp	1.0	82.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TTGCTACTGCGCTCTTCATT	50.0	45.0	57.6	57.9	323-476 bp	1.0	82.3
petN-psbM_p3	ATTCTAAGTCTCGCTTGGGC	GCTACTGCGCTCTTCATTCT	50.0	50.0	57.7	58.1	383-536 bp	1.0	82.1
petN-psbM_p4	ATTCTAAGTCTCGCTTGGGC	TTGCTACTGCGCTCTTCATT	50.0	45.0	57.7	57.9	385-538 bp	1.0	81.9
petN-psbM_p5	ATTCTAAGTCTCGCTTGGGC	ATTGCTACTGCGCTCTTCAT	50.0	45.0	57.7	57.7	386-539 bp	1.0	80.1