Hotspot

atpF-atpH

LSC · 11140-11511 · 372 bp

Back to Eremogone

Pi 0.0126

MarkerSeek score 69.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 11
pi: 0.0126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.0	57.3	531-547 bp	1.0	78.6
atpF-atpH_p2	TTAACCCCAAAACTCTCGGC	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.1	57.3	557-573 bp	1.0	77.9
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.0	58.0	526-542 bp	1.0	76.2
atpF-atpH_p4	TTAACCCCAAAACTCTCGGC	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.1	58.0	552-568 bp	1.0	75.5
atpF-atpH_p5	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTT	50.0	45.0	58.0	56.8	456-472 bp	1.0	75.5