Hotspot

rpoC2

LSC · 26553-31082 · 4530 bp

Back to Eremochloa

Pi 0.00324

MarkerSeek score 42.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4530
variable_sites: 22
pi: 0.00324
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTTGGCGAAAAAGCGAAGAA	CTGCCTCTTCGGGAAAATGA	45.0	50.0	57.9	57.9	881-914 bp	1.0	79.1
rpoC2_p2	GTTGGCGAAAAAGCGAAGAA	TCTGCCTCTTCGGGAAAATG	45.0	50.0	57.9	57.9	882-915 bp	1.0	79.1
rpoC2_p3	TAACGCCAGATCGAATCGTG	CTGCCTCTTCGGGAAAATGA	50.0	50.0	58.2	57.9	957-990 bp	1.0	78.6
rpoC2_p4	GAGGAAGAATATCGGACCCG	AGGCTCCCATATTCTTCCTCT	55.0	47.6	57.3	57.6	239 bp	1.0	65.3
rpoC2_p5	GAGGAAGAATATCGGACCCG	GGCTCCCATATTCTTCCTCTG	55.0	52.4	57.3	57.6	238 bp	1.0	64.9