Hotspot

ndhA

SSC · 113465-115583 · 2119 bp

Back to Eremochloa

Pi 0.00283

MarkerSeek score 56.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2119
variable_sites: 9
pi: 0.00283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGAGAACTTTTGTGTTGA	ATGGGGGAAGTTGATCGTTG	33.3	50.0	57.4	57.9	2191-2213 bp	1.0	43.6
ndhA_p2	TGTTTGAGAGAACTTTTGTGTTGA	GGGGGAAGTTGATCGTTGAA	33.3	50.0	57.4	57.8	2189-2211 bp	1.0	43.0
ndhA_p3	TCTTGTTTGAGAGAACTTTTGTGT	ATGGGGGAAGTTGATCGTTG	33.3	50.0	57.2	57.9	2194-2216 bp	1.0	41.6
ndhA_p4	TGAGAGAACTTTTGTGTTGAAACT	ATGGGGGAAGTTGATCGTTG	33.3	50.0	57.2	57.9	2187-2209 bp	1.0	41.6
ndhA_p5	TGAGAGAACTTTTGTGTTGAAACT	GGGGGAAGTTGATCGTTGAA	33.3	50.0	57.2	57.8	2185-2207 bp	1.0	41.1