Hotspot

atpF

LSC · 34636-36016 · 1381 bp

Back to Eremochloa

Pi 0.00434

MarkerSeek score 51.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1381
variable_sites: 9
pi: 0.00434
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACGAGCGAAGTAAGTGATCG	AGCAAGGGAAAAATAATGCCT	50.0	38.1	57.8	56.1	1509-1510 bp	1.0	55.7
atpF_p2	ACGAGCGAAGTAAGTGATCG	GAAGCAAGGGAAAAATAATGCCT	50.0	39.1	57.8	57.9	1511-1512 bp	1.0	54.4
atpF_p3	AAGGACGAGCGAAGTAAGTG	AGCAAGGGAAAAATAATGCCT	50.0	38.1	57.7	56.1	1513-1514 bp	1.0	54.3
atpF_p4	ACGAGCGAAGTAAGTGATCG	AAGCAAGGGAAAAATAATGCCT	50.0	36.4	57.8	56.8	1510-1511 bp	1.0	53.6
atpF_p5	CGAGCGAAGTAAGTGATCGA	AGCAAGGGAAAAATAATGCCT	50.0	38.1	57.5	56.1	1508-1509 bp	1.0	53.5