Hotspot

atpA

LSC · 36107-37630 · 1524 bp

Back to Eremochloa

Pi 0.00175

MarkerSeek score 46.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 4
pi: 0.00175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ATGAATCAGGTCCGACAACG	TCCCCGCTAAGAAGGTAGAG	50.0	55.0	58.0	57.9	2204-2209 bp	1.0	80.3
atpA_p2	ATGAATCAGGTCCGACAACG	ATGCATGTTTGTTACCGGGT	50.0	45.0	58.0	58.1	2392-2397 bp	1.0	80.1
atpA_p3	AAAGGGCGATGAATCAGGTC	TCCCCGCTAAGAAGGTAGAG	50.0	55.0	58.0	57.9	2212-2217 bp	1.0	80.0
atpA_p4	TATTGACACTCTCAGGGCCA	TCCCCGCTAAGAAGGTAGAG	50.0	55.0	58.0	57.9	2097-2099 bp	0.666667	65.8
atpA_p5	TTGACACTCTCAGGGCCATA	TCCCCGCTAAGAAGGTAGAG	50.0	55.0	58.0	57.9	2095-2097 bp	0.666667	65.8