Hotspot

trnN-GUU-rps15

IRb · 102599-103750 · 1152 bp

Back to Eremochloa

Pi 0.00116

MarkerSeek score 59.50

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1152
variable_sites: 2
pi: 0.00116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	ACGACCAGTCAGTTAACAGC	AATGTGAAGCAAGTCTCCGT	50.0	45.0	57.8	57.4	1296 bp	1.0	74.5
trnN-GUU-rps15_p2	AGGATTCGAACCTACGACCA	AATGTGAAGCAAGTCTCCGT	50.0	45.0	58.2	57.4	1309 bp	1.0	74.5
trnN-GUU-rps15_p3	GGATTCGAACCTACGACCAG	AATGTGAAGCAAGTCTCCGT	55.0	45.0	57.8	57.4	1308 bp	1.0	74.1
trnN-GUU-rps15_p4	ACCACTGAGCTACTGAGGAA	AATGTGAAGCAAGTCTCCGT	50.0	45.0	57.7	57.4	1266 bp	1.0	73.2
trnN-GUU-rps15_p5	CCCCAAGTAGGATTCGAACC	AATGTGAAGCAAGTCTCCGT	55.0	45.0	57.7	57.4	1317 bp	1.0	73.1