Hotspot

rpl20-rps12

LSC · 68104-68797 · 694 bp

Back to Eremochloa

Pi 0.00581
MarkerSeek score 63.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
694
variable_sites
6
pi
0.00581
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGCATTTTTGTTCGTCGTC GAGGAACATGTGCTAGGGTG 45.0 55.0 58.0 58.0 766-771 bp 1.0 80.8
rpl20-rps12_p2 ACGCATTTTTGTTCGTCGTC CGACATGCCAACGGTTAAAC 45.0 50.0 58.0 58.0 861-866 bp 1.0 80.6
rpl20-rps12_p3 ACGCATTTTTGTTCGTCGTC GTCGAGGAACATGTGCTAGG 45.0 55.0 58.0 58.1 769-774 bp 1.0 80.5
rpl20-rps12_p4 GTTTGAGGCAAATGAACGCA GAGGAACATGTGCTAGGGTG 45.0 55.0 57.9 58.0 781-786 bp 1.0 79.8
rpl20-rps12_p5 GTTTGAGGCAAATGAACGCA CGACATGCCAACGGTTAAAC 45.0 50.0 57.9 58.0 876-881 bp 1.0 79.6