Hotspot

psaI-ycf4

LSC · 58908-59267 · 360 bp

Back to Eremochloa

Pi 0.00743

MarkerSeek score 52.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 4
pi: 0.00743
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TTTCCGGCAATTGCAATGAC	CCCCAACCGCTAAGAATCC	45.0	57.9	57.9	57.9	537-538 bp	1.0	71.4
psaI-ycf4_p2	TTTCCGGCAATTGCAATGAC	TCCATATGTGTTCTGATCGCC	45.0	47.6	57.9	57.9	447-448 bp	1.0	71.4
psaI-ycf4_p3	TTTCCGGCAATTGCAATGAC	CCATATGTGTTCTGATCGCCA	45.0	47.6	57.9	58.2	446-447 bp	1.0	71.0
psaI-ycf4_p4	TTTCCGGCAATTGCAATGAC	TGTGTTCTGATCGCCAATTCA	45.0	42.9	57.9	58.2	441-442 bp	1.0	70.5
psaI-ycf4_p5	TTTCCGGCAATTGCAATGAC	CCCAACCGCTAAGAATCCTA	45.0	50.0	57.9	56.7	536-537 bp	1.0	69.8