Hotspot

rbcL-psaI

LSC · 57835-58796 · 962 bp

Back to Eremochloa

Pi 0.00762

MarkerSeek score 58.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 11
pi: 0.00762
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	1120-1130 bp	1.0	80.7
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.2	57.9	1099-1109 bp	1.0	79.5
rbcL-psaI_p3	TGCAAATGGAGTGCTGAACT	GAAGTCATTGCAATTGCCGG	45.0	50.0	58.0	58.4	1123-1133 bp	1.0	78.4
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	GGAAGTCATTGCAATTGCCG	45.0	50.0	58.0	58.4	1124-1134 bp	1.0	78.4
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	GAAGTCATTGCAATTGCCGG	50.0	50.0	58.2	58.4	1102-1112 bp	1.0	77.2